Predicting manual wheelchair initiation movement with EMG activity during over ground propulsion.

Context/Objective: This is a preliminary study of movement finalities prediction in manual wheelchairs (MWCs) from electromyography (EMG) data. MWC users suffer from musculoskeletal disorders and need assistance while moving. The purpose of this work is to predict the direction and speed of movement in MWCs from EMG data prior to movement initiation. This prediction could be used by MWC to assist users in their displacement by doing a smart electrical assistance based on displacement prediction.Design: Experimental study.Setting: Trained Subject LAMIH Laboratory.Participants: Eight healthy subjects trained to move in manual wheelchairs.Interventions: Subjects initiated the movement in three directions (front, right and left) and with two speeds (maximum speed and spontaneous speed) from two hand positions (on the thighs or on the handrim). A total of 96 movements was studied. Activation of 14 muscles was recorded bilaterally at the deltoid anterior, deltoid posterior, biceps brachii, pectoralis major, rectus abdominis, obliquus externus and erector spinae.Outcome Measures: Prior amplitude, prior time and anticipatory postural adjustments were measured. A hierarchical multi-class classification using logistic regression was used to create a cascade of prediction models. We performed a stepwise (forward-backward) selection of variables using the Bayesian information criterion. Percentages of well-classified movements have been measured through the means of a cross-validation.Results: Prediction is possible using the EMG parameters and allows to discriminate the direction / speed combination with 95% correct classification on the 6 possible classes (3 directions * 2 speeds).Conclusion: Action planning in the static position showed significant adaptability to the forthcoming parameters displacement. The percentages of prediction presented in this work make it possible to envision an intuitive assistance to the initiation of the MWC displacement adapted to the user's intentions.

Antibiotic export by MexB multidrug efflux transporter is allosterically controlled by a MexA-OprM chaperone-like complex.

The tripartite multidrug efflux system MexAB-OprM is a major actor in Pseudomonas aeruginosa antibiotic resistance by exporting a large variety of antimicrobial compounds. Crystal structures of MexB and of its Escherichia coli homolog AcrB had revealed asymmetric trimers depicting a directional drug pathway by a conformational interconversion (from Loose and Tight binding pockets to Open gate (LTO) for drug exit). It remains unclear how MexB acquires its LTO form. Here by performing functional and cryo-EM structural investigations of MexB at various stages of the assembly process, we unveil that MexB inserted in lipid membrane is not set for active transport because it displays an inactive LTC form with a Closed exit gate. In the tripartite complex, OprM and MexA form a corset-like platform that converts MexB into the active form. Our findings shed new light on the resistance nodulation cell division (RND) cognate partners which act as allosteric factors eliciting the functional drug extrusion.

Minimal nanodisc without exogenous lipids for stabilizing membrane proteins in detergent-free buffer.

Membrane protein stabilization after detergent solubilization presents drawbacks for structural and biophysical studies, in particular that of a reduced stability in detergent micelles. Therefore, alternative methods are required for efficient stabilization. Lipid nanodisc made with the membrane scaffold protein MSP is a valuable system but requires a fine optimization of the lipid to protein ratio. We present here the use of the scaffold protein MSP without added lipids as a minimal system to stabilize membrane proteins. We show that this method is applicable to α-helical and ß-strands transmembrane proteins. This method allowed cryo-electron microscopy structural study of the bacterial transporter MexB. A protein quantification indicates that MexB is stabilized by two MSP proteins. This simplified and efficient method proposes a new advance in harnessing the MSP potential to stabilize membrane proteins.

Arm-trunk coordination in wheelchair initiation displacement: A study of anticipatory and compensatory postural adjustments during different speeds and directions of propulsion.

Arm-trunk coordination during the initiation of displacement in manual wheelchair is a complex task. The objective of this work is to study the arm-trunk coordination by measuring anticipatory and compensatory postural adjustments. Nine healthy subjects participated in the study after being trained in manual wheelchair. They were asked to initiate a displacement in manual wheelchair in three directions (forward vs. left vs. right), with two speeds (spontaneous vs. maximum) and with two initial hand's positions (hands on thighs vs. hands on handrails). Muscular activities in the trunk (postural component) and the arms (focal component) were recorded bilaterally. The results show two strategies for trunk control: An anticipatory adjustment strategy and a compensatory adjustment strategy with a dominance of compensation. These two strategies are influenced by the finalities of displacement in terms of speed and direction depending on the hands positions. Arm-trunk coordination is characterized by an adaptability of anticipatory and compensatory postural adjustments. The study of this type of coordination for subjects with different levels of spinal cord injury could be used to predict the forthcoming displacement and thus assist the user in a complex task.

An index to quantify deviations from normal trunk mobility: Clinical correlation and initial test of validity.

BACKGROUND: In case of people suffering from chronic low back pain, specific movements of the hip, pelvis, and trunk are associated with pain. Comparing range of motion measurements for multiple planes and from different segments and lines in reference to those of healthy individuals seems interesting but present interpretations challenge in relation to important number of variables and correlation with clinical data. METHODS: The proposed index is based on using principal component analysis to quantify differences in trunk mobility between patients with chronic low back pain and a control group. Kinematic data were recorded for the cervical and thoracic vertebrae, the lumbar spine, and the pelvic and scapular belts during repeated trials (hip flexion and extension, hip bending, and trunk twists). Angular motion values were calculated. Principal component analysis was used to convert 10 discrete variables (kinematical data) extracted from control data into 10 independent variables. FINDINGS: The proposed index comprises the sum of the variables. Initial demonstration of its clinical utility and statistical tests of this index validity were revealed. It establishes correlations between the psychosocial impact of chronic low back pain, trunk mobility (as summarized by the index) and the positive effects of functional restoration program. INTERPRETATION: This index let to assess the absolute potential benefits of rehabilitation in term of kinematic motion. Functional restoration program promotes the physical functioning of patients by increasing their range of motion. This index uses kinematic motion to assess the potential benefits of such rehabilitation program.

Xenon for tunnelling analysis of the efflux pump component OprN.

Tripartite efflux pumps are among the main actors responsible for antibiotics resistance in Gram-negative bacteria. In the last two decades, structural studies gave crucial information about the assembly interfaces and the mechanistic motions. Thus rigidifying the assembly seems to be an interesting way to hamper the drug efflux. In this context, xenon is a suitable probe for checking whether small ligands could act as conformational lockers by targeting hydrophobic cavities. Here we focus on OprN, the outer membrane channel of the MexEF efflux pump from Pseudomonas aeruginosa. After exposing OprN crystals to xenon gas pressure, 14 binding sites were observed using X-ray crystallography. These binding sites were unambiguously characterized in hydrophobic cavities of OprN. The major site is observed in the sensitive iris-like region gating the channel at the periplasmic side, built by the three key-residues Leu 405, Asp 109, and Arg 412. This arrangement defines along the tunnel axis a strong hydrophobic/polar gradient able to enhance the passive efflux mechanism of OprN. The other xenon atoms reveal strategic hydrophobic regions of the channel scaffold to target, with the aim to freeze the dynamic movements responsible of the open/close conformational equilibrium in OprN.

Benefits of physical activities centered on the trunk for pregnant women.

OBJECTIVE: To examine the effect of a trunk exercise program on pain, quality of life (QoL) and physical health (PH) in the late pregnancy and post-partum periods as well as baby weight and size and delivery. METHODS: 90 nulliparous women were allocated based on voluntarism to a training group (TG) or to a control group (CG). TG carried out a structured program with exercises for flexibility, balance and strengthening for the majority of skeletal muscles specifically for the spinal ones, between the 24th and 36th week of pregnancy. Both TG and CG were evaluated for pain at the beginning of the program (T1), at the end of the program (T2) and two months' post-partum (T3). QoL was assessed at T1,T2 and T3, and PH at T1 and T3. Mann-Whitney test was utilized to compare TG and CG on each period and to compare the periods two by two. RESULTS: At T1, no significant difference was found between the two groups in pain intensity (pain interference p = 0.317), QoL (p = 0.18) and PH (flexibility p = 0.06; walking p = 0.85). At T2, women of TG had a lower intensity of pain than CG (legs (p = 0.029), lower back (p < 0.0001), upper back (p = 0.022), pelvis (p = 0.017), groins (p = 0.043), lower pelvis (p = 0.009) and interference of pain (p < 0.0001)). At T3, TG had a lower intensity of pain than CG, in lower back and upper back (p < 0.0001) and interference of pain (p < 0.0001). Best scores of QoL were observed in TG compared to CG at T2 (p < 0.0001) and at T3 (p < 0.0001). PH in TG compared to CG was not different between the groups in T1 but was different in in T3 (flexibility p = 0.002, walking, balance, curling-ups and Ruffier p < 0.0001). TG were four times less likely to have a caesarean (p = 0.049) and had 4.5 h less of labor time (p < 0.0001). No difference was observed between the TG vs CG concerning the weight (p = 0.22) and the baby's size (p = 0.11). CONCLUSION: Strengthening exercises centered on the trunk reduce pain, improve QoL and PH in late pregnancy and at two months in the post-partum period. Strengthening exercises also ease the delivery.

Reorganization of motor cortex and impairment of motor performance induced by hindlimb unloading are partially reversed by cortical IGF-1 administration.

Immobilization, bed rest, or sedentary lifestyle, are known to induce a profound impairment in sensorimotor performance. These alterations are due to a combination of peripheral and central factors. Previous data conducted on a rat model of disuse (hindlimb unloading, HU) have shown a profound reorganization of motor cortex and an impairment of motor performance. Recently, our interest was turned towards the role of insulin-like growth factor 1 (IGF-1) in cerebral plasticity since this growth factor is considered as the mediator of beneficial effects of exercise on the central nervous system, and its cortical level is decreased after a 14-day period of HU. In the present study, we attempted to determine whether a chronic subdural administration of IGF-1 in HU rats could prevent deleterious effects of HU on the motor cortex and on motor activity. We demonstrated that HU induces a shrinkage of hindlimb cortical representation and an increase in current threshold to elicit a movement. Administration of IGF-1 in HU rats partially reversed these changes. The functional evaluation revealed that IGF-1 prevents the decrease in spontaneous activity found in HU rats and the changes in hip kinematics during overground locomotion, but had no effect of challenged locomotion (ladder rung walking test). Taken together, these data clearly indicate the implication of IGF-1 in cortical plastic mechanisms and in behavioral alteration induced by a decreased in sensorimotor activity.

Adaptability and Prediction of Anticipatory Muscular Activity Parameters to Different Movements in the Sitting Position.

Voluntary movement often causes postural perturbation that requires an anticipatory postural adjustment to minimize perturbation and increase the efficiency and coordination during execution. This systematic review focuses specifically on the relationship between the parameters of anticipatory muscular activities and movement finality in sitting position among adults, to study the adaptability and predictability of anticipatory muscular activities parameters to different movements and conditions in sitting position in adults. A systematic literature search was performed using PubMed, Science Direct, Web of Science, Springer-Link, Engineering Village, and EbscoHost. Inclusion and exclusion criteria were applied to retain the most rigorous and specific studies, yielding 76 articles, Seventeen articles were excluded at first reading, and after the application of inclusion and exclusion criteria, 23 were retained. In a sitting position, central nervous system activity precedes movement by diverse anticipatory muscular activities and shows the ability to adapt anticipatory muscular activity parameters to the movement direction, postural stability, or charge weight. In addition, these parameters could be adapted to the speed of execution, as found for the standing position. Parameters of anticipatory muscular activities (duration, order, and amplitude of muscle contractions constituting the anticipatory muscular activity) could be used as a predictive indicator of forthcoming movement. In addition, this systematic review may improve methodology in empirical studies and assistive technology for people with disabilities.

Using EMGs and kinematics data to study the take-off technique of experts and novices for a pole vaulting short run-up educational exercise.

This study attempts to characterise the electromyographic activity and kinematics exhibited during the performance of take-off for a pole vaulting short run-up educational exercise, for different expertise levels. Two groups (experts and novices) participated in this study. Both groups were asked to execute their take-off technique for that specific exercise. Among the kinematics variables studied, the knee, hip and ankle angles and the hip and knee angular velocities were significantly different. There were also significant differences in the EMG variables, especially in terms of (i) biceps femoris and gastrocnemius lateralis activity at touchdown and (ii) vastus lateralis and gastrocnemius lateralis activity during take-off. During touchdown, the experts tended to increase the stiffness of the take-off leg to decrease braking. Novices exhibited less stiffness in the take-off leg due to their tendency to maintain a tighter knee angle. Novices also transferred less energy forward during take-off due to lack of contraction in the vastus lateralis, which is known to contribute to forward energy transfers. This study highlights the differences in both groups in terms of muscular and angular control according to the studied variables. Such studies of pole vaulting could be useful to help novices to learn expert's technique.

A 3D analysis of fore- and hindlimb motion during overground and ladder walking: comparison of control and unloaded rats.

During locomotion, muscles are controlled by a network of neurones located in the spinal cord and by supraspinal structures. Alterations in that neuromuscular system have a functional impact, in particular on locomotion. The hindlimb unloading (HU) model in rat has been commonly used to generate disuse since it suppresses the hindlimb loading and limits movements. In consequence, it induces plastic mechanisms in the muscle, the spinal cord and the sensorimotor cortex. The aim of this study was to assess the locomotion in HU rats in two conditions: (1) on a runway and (2) in a challenging situation involving the participation of supraspinal structures (ladder walking). For that purpose, the motor pattern has been investigated by means of 3D motion analysis of the right fore- and hindlimbs as well as electromyographic recording of the soleus and tibialis anterior muscles. The 3D motion results show that HU induces a support-dependent alteration of the kinematics: increased duration of step, stance and swing; increased ankle flexion during stance and hyperextension at toe-off; lower protraction during swing. The electromyographic results show that whatever the support, the flexor and extensor burst duration was longer in HU rats. In addition, results show that ladder exacerbates some effects of HU. As ladder walking is a situation which requires precision, it is suggested that the control of hindlimb movement by supraspinal structures is affected in HU rats.

A 3D analysis of fore- and hindlimb motion during locomotion: comparison of overground and ladder walking in rats.

The locomotor pattern, generated by the central pattern generator, is under the dependence of descending and peripheral pathways. The afferent feedback from peripheral receptors allows the animal to correct for disturbances that occur during walking, while supraspinal structures are important for locomotion in demanding situations such as ladder walking. Such walking, by regards to the control needed for accuracy of movements, is now widely used for description of consequences of nervous system dysfunction on motor performance. It is important to have a good knowledge of the changes in kinematic parameters according to walking conditions, since it might reflect different neural mechanisms. The aim of this work was to perform a 3D kinematic analysis of both hind- and forelimb during overground and ladder walking, to study qualitative and quantitative locomotor characteristics in different modes of locomotion. The analysis was performed on 5 rats. Movements of the right hind- and forelimb were evaluated using a 3D optical analyser, and EMG of the soleus and tibialis anterior muscles was synchronously recorded. Results indicate that kinematic and electromyographic characteristics of locomotion are dependent on the type of support. Changes were more obvious for hindlimb than for forelimb. Velocity, stride length and tibialis anterior burst duration were lower on ladder than on runway. In addition, during ladder walking, a protraction was noticed, rats bring their feet more rostral at the end of the swing phase. All these changes constitute an adaptive strategy to allow a better tactile activity with forelimbs and to avoid foot misplacement.

A 3D analysis of hindlimb motion during treadmill locomotion in rats after a 14-day episode of simulated microgravity.

This study describes the effect of simulated microgravity in rat on kinematics and electromyographic activity during treadmill locomotion. The analysis was performed in rats submitted to 14 days of hindlimb unloading (HU), in rats submitted to hindlimb unloading and then authorized to recover for 7 days (REC), and in aged-matched control rats (CON). Movements of the right hindlimb were measured with a 3D-optical analyzer (SAGA3 system) and five small infrared-reflective disks positioned on the skin, recorded by three CCD cameras. Results showed that HU rats exhibited hyperextensions at the end of the stance phase. By contrast, during the major part of the step, the ankle was less extended than CON. Possible origins of the changes are discussed. This leads to the question of how important is sensory input in the regulation of the locomotor pattern after HU. Data obtained in REC animals showed that 1 week of recovery allowed the restoration of a good locomotor performance. However, the limb motion remained abnormal, and at contrary to HU rats: higher extension during the step, except at push-off when the limb was in hyperflexion. We concluded that simulated microgravity involves a dual adaptive process: a first one during unloading, and a second one during the period of recovery, which is not a simple return to initial characteristics of the locomotor pattern.